COMPARISON BETWEEN SALIVARY FERNING AND VAGINAL CYTOLOGY IN RELATION TO CONCEPTION IN ISLAND-BRED DOGS (Canis familiaris)
Friday, October 18, 2019, 2:30 pm – 3:15 pm
Reuter King M. Bernardo, Nicole Anne G. Ole, Rey B. Oronan, Marco F. Reyes
Department of Veterinary Clinical Sciences, College of Veterinary Medicine, University of the Philippines Los Baños, Laguna, Philippines
Salivary ferning has been used to detect ovulation in animals and humans. Saliva is composed of complex mixtures of enzymes, mucins, antibodies, electrolytes, and hormones. To date, studies and research on saliva ferning in determining ovulation time in bitches in the country is nil. A survey of literature proves that there is no local information and data regarding the comparison of saliva ferning and vaginal cytology
A total of 43 paired samples of saliva and vaginal smear were conveniently sampled. The vaginal smears were routinely examined and saliva samples were observed for the presence of fern-like patterns and then graded depending on the degree of presence and length of stems, venations, and subventions. Following the recommendations, 48 female dogs in estrus were tested using salivary ferning and vaginal cytology until the peak of ferning was observed. A total of 96 samples consisting of saliva and vaginal swab were obtained from the subjects and examined under the microscope. Breeding was recommended two or three days after observing the ferning scores of 4 or 5. After 45 to 55 days of the last date of breeding, ultrasonography was performed to detect pregnancy.
Based on these results, salivary ferning may be a more dependable method although specificity could have been higher. The conception rates based on salivary ferning and vaginal cytology were 67.50% and 68.18%, respectively. Salivary ferning can serve as a potential diagnostic tool for estrus detection given that its association with conception has been established and its sensitivity and accuracy were higher than vaginal cytology.